Plasmid

Part:BBa_K3600014:Design

Designed by: yasmine koubaa   Group: iGEM20_EPFL   (2020-10-27)


Lys2-TRX2pr-mScarleti cassette


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1034
    Illegal EcoRI site found at 1279
    Illegal EcoRI site found at 1859
    Illegal XbaI site found at 979
    Illegal XbaI site found at 1288
    Illegal XbaI site found at 2036
    Illegal SpeI site found at 2875
    Illegal PstI site found at 2884
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1034
    Illegal EcoRI site found at 1279
    Illegal EcoRI site found at 1859
    Illegal SpeI site found at 2875
    Illegal PstI site found at 2884
    Illegal NotI site found at 763
    Illegal NotI site found at 3439
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal suffix found in sequence at 2606
    Illegal EcoRI site found at 1034
    Illegal EcoRI site found at 1279
    Illegal EcoRI site found at 1859
    Illegal BglII site found at 2477
    Illegal BglII site found at 2899
    Illegal BamHI site found at 2911
    Illegal XhoI site found at 2920
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1034
    Illegal EcoRI site found at 1279
    Illegal EcoRI site found at 1859
    Illegal XbaI site found at 979
    Illegal XbaI site found at 1288
    Illegal XbaI site found at 2036
    Illegal SpeI site found at 2875
    Illegal PstI site found at 2884
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1034
    Illegal EcoRI site found at 1279
    Illegal EcoRI site found at 1859
    Illegal XbaI site found at 979
    Illegal XbaI site found at 1288
    Illegal XbaI site found at 2036
    Illegal SpeI site found at 2875
    Illegal PstI site found at 2884
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 796


Design Notes

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Source

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References